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KMID : 0368420040470020111
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Journal of Plant Biology
2004 Volume.47 No. 2 p.111 ~ p.116
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Increased tolerance to methyl viologen by transgenic tobacco plants that over-express the cytosolic glutathione reductase gene fromBrassica campestris
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Lee Hyo-Shin
Jo Jin-Ki
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Abstract
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We placedBcGR1, a Chinese cabbage (Brassica campestris var.Pekinensis) gene that encodes cytosolic glutathione reductase (GR), under the control of the cauliflower mosaic virus (CaMV) 35S promoter. This gene was then introduced into tobacco plants viaAgrobacterium-mediated transformation. Segregation and northern analyses of the transgenic plants revealed no relationship between the copy number of the introduced gene and levels ofBcGR1 transcript. Homozygous lines containingBcGR1 were generated and tested for their acquisition of increased tolerance to oxidative stress. When 10-d-old transgenic tobacco seedlings were treated with 5 to 20 μM methyl viologen (MV), they showed significantly increased tolerance compared with the wild-type seedlings. The most drastic difference was observed at a concentration of 10 μM MV. In addition, when leaf discs were subjected to MV, the transgenic plants were less damaged than the wild types with regard to their electrical conductivity and chlorophyll content.
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KEYWORD
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Brassica campestris, glutathione reductase, methyl viologen, oxidative stress, transgenic tobacco plant
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